Journal: bioRxiv

Article Title: A 50-gene high-risk profile predictive of COVID-19 and Idiopathic Pulmonary Fibrosis mortality originates from a genomic imbalance in monocyte and T-cell subsets that reverses in survivors with post-COVID-19 Interstitial Lung Disease

doi: 10.1101/2023.10.22.563156

Figure Lengend Snippet: A . Study design of the 50-gene signature and cytokine analysis in COVID-19 patients (Cohort 1). B. Heatmap of COVID-19 patients based on the 50-gene signature discriminates three risk groups (low, intermediate, and high) based on SAMS. Every column represents a patient, and every row represents a gene. Log-based two-color scale is adjacent to the heatmap. Red denotes increased expression and green denotes decreased expression. Gene expression data is represented as Log2 normalized expression values. C-D. Time to death and time to discharge by day 60 in hospitalized patients with COVID-19, respectively. E-H. Plasma cytokine concentrations (IL6, IP-10, SPP1 and TGFβ) in low, intermediate, and high-risk profile patients with COVID-19 at days 2, 6 and 13 post admission. The data is presented as an average of triplicate values ± SEM for each group. Two-way ANOVA test (GraphPad software) Tukey’s multiple comparisons were used; * p<0.05. I. Study design of 7-gene signature analysis by RT-qPCR in PBMCs from COVID-19 patients (Cohort 2). J. Heatmap of COVID-19 patients based on the 50-gene signature discriminates three risk groups (low, intermediate, and high) based on SAMS Up scores. Heatmap nomenclature is the same as in . K-L. Time to death and time to discharge by day 60 in hospitalized patients with COVID-19 respectively in cohort two. The data is presented as an average of triplicated TUs values ± SEM for each group. * p<0.05

Article Snippet: We measured cytokine concentrations of 121 plasma samples from COVID-19 patients from Cohort 1 using a customized, Bioplex 200 compatible, human cytokine panel including, IL6, IP10, SPP1 and TGFβ-1 (#FCSTM18-06, R&D Systems).

Techniques: Expressing, Software, Quantitative RT-PCR

Journal: bioRxiv

Article Title: Toward Improving Immunotolerance for Stem Cell-Derived Islets

doi: 10.1101/2022.09.15.508091

Figure Lengend Snippet: Reconstitution of the immune tolerant environment using cytokine cocktails. A. Cytokine array of secreted cytokines and chemokines in testis supernatant. The spots on the top left, top right and bottom left were the reference spots. B. Quantification of cytokines and chemokines normalized to the blank medium control. C. IL-2 and IL-10 Elisa of testis supernatant confirm secretion of cytokines in the testis. D. Cytokines screening for induction of T regs in by CD3/CD28 beads. 3 days after expansion, MNCs were collected for CD25 and CD4 staining. E. T cell stimulated by CD3/CD28 beads while adding IL-2, IL-10, TGFβ, or a combination of cytokines, measured 3 days after co-culture. Upper row, T regs induction under each condition. Lower row, T cell proliferation tested by CFSE cell proliferation trace under each condition. F. Immunostaining of IL-2, INS, FOXP3, and CD8 in grafts at 10 days in the control group, and 2, 4, and 8 weeks show a protective effect of the cytokine cocktails (bar = 100 μm).

Article Snippet: 48 hours after culturing, the testis supernatant and the blank medium were collected for the cytokine array (R&D System, ARY006).

Techniques: Control, Enzyme-linked Immunosorbent Assay, Staining, Co-Culture Assay, Immunostaining